The sample (N=203) was divided into two groups. Results showed that GDFMs scored significantly higher than non GDFMs (i) on anxiety and depression scales, (ii) on difficulties in emotion regulation, and (iii) on maladaptive coping strategies. Additionally, difficulties in emotion regulation and coping strategies correlated with anxiety and depression.
steroid Glutamine synthetase (GS) steroids, the enzyme responsible for the first step in the assimilation of ammonium in higher plants, generally exists in a number of isoforms associated with different tissues or cellular compartments within the plant. This study has investigated the GS isoenzyme composition of roots and N fixing root nodules of the temperate legume, Lotus comiculatus, and has used a novel transgenic approach to manipulate the spatial distribution of the enzyme within these tissues.The isoforms of GS were studied using ion exchange chromatography and western blotting, and a nodule specific isoform was identified. Nitrate treatment of the N2 fixing plants had a marked effect on the nodule isoform, converting it to a form that was indistinguishable by ion exchange chromatography from the root isoenzyme.To allow tissue specific manipulation of GS activity steroids, a chimaeric gene was constructed consisting of a translational fusion between the pat and uidA genes coding for phosphinothricin acetyl transferase (PAT) and P glucuronidase (GUS), respectively. steroid
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